Contribution by: F. García-Breijo, J. Reig-Armiñana & V. Calatayud

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Pistacia terebinthus - Microscopic Injury

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Common name: Terebinth

Microscopic symptoms induced in controlled conditions (fumigation with ozone)

Before visible symptoms are externally evident, some alterations can be observed by microscopy (photo below): the vacuolar content of the palisade parenchyma cells (PP) shows the aspect of bands which stain differently; walls are altered (arrows), intercellular spaces (IS) increase in both the palisade (PP) and spongy parenchyma (SP); Inner tangential walls of occlusive cells of stomata (S) increase their thickness.

Before visible symptoms are externally evident, some alterations can be observed by microscopy (photo below): mature leaf stained with toluidine blue (4 weeks in the NF+40 treatment; x 1000); Phloem (Ph) cells surround two secretory canals (SC); Content of the phloem cells becomes more dense, and some cells are filled with tannins; Cells lining the secretory canal lumen are not turgent and some are degraded (arrows).

Before visible symptoms are externally evident, some alterations can be observed by microscopy (photo below): callose deposition in the phloem (white fluorescent dots) (4 weeks in the NF+40 treatment; x1000).

Visible symptoms induced in controlled conditions (fumigation with ozone)

Fumigation of Pistacia terebinthus with enhanced ozone levels in Open Top Chambers (OTCs) induced brown stippling, with relatively large dots, sometimes with associated chlorosis of the older leaves.

© 2011 Fundación CEAM © 2011 Copyright of the photos: their authors